What is caspase Glo assay?
The Caspase-Glo® 3/7 Assay is a luminescent assay that measures caspase-3 and -7 activities in purified enzyme preparations or cultures of adherent or suspension cells. The assay provides a proluminescent caspase-3/7 substrate, which contains the tetrapeptide sequence DEVD.
What is a caspase-3 assay?
The Caspase-3 assay protocol is based on the formation of the chromophore p-nitroaniline (p-NA) by cleavage from the labeled substrate DEVD-pNA. The p-NA can be quantified using a spectrophotometer or a microtiter plate reader reading absorbance at 400 or 405 nm.
How does caspase-3 work?
Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular structures such as DNA fragmentation or degradation of cytoskeletal proteins (1). The activity of caspase-3 is tightly regulated and it is produced as zymogen in an inactive pro-form (1).
What is the difference between ATP assay and tetrazolium reduction assay?
That difference provides the basis for many of the commonly used cell viability assays. The ATP assay is somewhat different in that the addition of assay reagent immediately ruptures the cells, thus there is no incubation period of reagent with a viable cell population. Tetrazolium Reduction Assays
What is the difference between ATP assay and cell viability assay?
That difference provides the basis for many of the commonly used cell viability assays. The ATP assay is somewhat different in that the addition of assay reagent immediately ruptures the cells, thus there is no incubation period of reagent with a viable cell population.
What are the different assay methods used in cell culture?
The assay methods covered include the use of different classes of colorimetric tetrazolium reagents, resazurin reduction and protease substrates generating a fluorescent signal, the luminogenic ATP assay, and a novel real-time assay to monitor live cells for days in culture.
What is a viable cell number assay used for?
The assays described are based on measurement of a marker activity associated with viable cell number. These assays are used for measuring the results of cell proliferation, testing for cytotoxic effects of compounds, and for multiplexing as an internal control to determine viable cell number during other cell-based assays. NCBI