What is arithmetic method of Reed and Muench?
The Reed–Muench method is a simple method for determining 50% endpoints in experimental biology, that is, the concentration of a test substance that produces an effect of interest in half of the test units.
What is endpoint dilution assay?
The end-point dilution assay was used to measure virus titer before the development of the plaque assay, and is still used for viruses that do not form plaques. Serial dilutions of a virus stock are prepared and inoculated onto replicate cell cultures, often in multi-well formats (e.g. 96 well plastic plates).
How do you calculate Moi from PFU ml?
For example: You have a virus with a titer of 1.3×1011 PFU/ml and a well that contains 1.8×106 cells. You want to make that well contain 200 MOI. Therefore, formula 1: (1.8×106 cells ) * (200 MOI) = 3.6×108 PFU desired. Then, formula 2: (3.6×108 PFU desired) / (1.3×1011 PFU/ml) = 0.0028 ml or 2.8l.
How do you convert TCID50 ml to PFU ml?
The titer as measured by TCID50 is 0.7 Log higher than the titer by standard plaque assay. To transform TCID50/ml into PFU/ml: T = 1 X 108.
How is PFU calculated?
Calculating PFU Divide the number of plaques by the dilution factor, (ex. 10-6 for the most diluted sample) toobtain the number of Plaque Forming Units (PFU) in 100 μL of phage mixture. Note: If performing the assay in triplicate, use the average number of plaques from the three plates.
What are the three methods used to detect a virus?
Virus Detection Methods Top There are four major methods of virus detection in use today: scanning, integrity checking, interception, and heuristic detection. Of these, scanning and interception are very common, with the other two only common in less widely-used anti-virus packages.
How do you find the MOI of AAV?
For an MOI = 1, the volume (in µl) of AAV particles needed = ((total number of cells per well)/(number of genome copies (GC)/ml)) x 1,000. The MOI used is critical to achieve 100% infection of the target cells without causing major side effects.
How do you calculate titer MOI?
For example, assuming the titer (functional titer) of the viral particles for a pooled screen is 1 x 10^7 TU/ml (thus 1 x 10^4 TU/µl etc.), for 1 x 10^7 cells, 300 µl of the viral suspension needs to be added to achieve an MOI=0.3.
How is MOI AAV calculated?